Nico Stuurman is a Research Specialist at the University of California, San Francisco, in the lab of Ron Vale. Nico combines his expertise in computer programming and microscopy to advance many projects including the Open Source software, Micro-Manager.
Talks with this Speaker
Introduction to Image Acquisition for Quantitative Analysis
Nico Stuurman provides an overview of the different tools, equipment, and software available to acquire an image of a biological sample, and the considerations one needs to take when using these tools. (Talk recorded in October 2018)
Audience:
- Researcher
- Educators of Adv. Undergrad / Grad
Duration: 00:29:35
Cameras and Digital Image Analysis
Nico Stuurman describes how digital cameras for microscopes work, what a pixel is, Nyquist sampling, the dynamic range, noise, color cameras, and digital image analysis. (Talk recorded in April 2013)
Audience:
- Student
- Researcher
- Educators of Adv. Undergrad / Grad
Duration: 33:40
Introduction to Fluorescence Microscopy
In this introductory lecture on light microscopy, Dr. Nico Stuurman describes the principles and properties of fluorescence microscopy. (Talk recorded in April 2012)
Audience:
- Student
- Researcher
- Educators of Adv. Undergrad / Grad
Duration: 33:34
High Speed Synchronization
Nico Stuurman shows how hardware synchronization can dramatically speed up microscope image acquisition and reduce light exposure to biological samples. (Talk recorded in April 2012)
Audience:
- Researcher
Duration: 5:50
Camera Calibration
In this video about camera calibration, Nico Stuurman shows you the steps you need to take to make sure your camera is working properly. (Talk recorded in April 2012)
Audience:
- Researcher
Duration: 19:31
Using Software to Control Microscopes
In this talk about using software to control microscopes, Nico Stuurman illustrates some basic principles used in all microscope software packages. (Talk recorded in April 2012)
Audience:
- Researcher
Duration: 11:33
Cameras and Detectors II: Specifications and Performance
This second lecture on detectors discusses how to match the pixel size of your camera to the optical resolution of the microscope, and it touches on quantum efficiency and its improvement by back-illumination. (Talk recorded in April 2013)
Audience:
- Researcher
Duration: 36:25
Cameras and Photosensitive Detectors I: How Do They Work?
Nico Stuurman introduces the two main classes of photosensitive detectors, single-point and multi-point detectors, and explains their basic principles. (Talk recorded in April 2012)
Audience:
- Researcher
Duration: 22:56
Demystifying Microscopes: Disassembling an ASI RAMM Microscope
Nico Stuurman shows us the internals of the ASI RAMM microscope by disassembling the microsope into its separate components and explaining what each part does. (Talk recorded in January 2013)
Audience:
- Researcher
Duration: 18:12
Introduction to Fluorescence Microscopy
In this introductory lecture on light microscopy, Dr. Nico Stuurman describes the principles and properties of fluorescence microscopy. (Talk recorded in April 2012)
Audience:
- Student
- Researcher
- Educators of Adv. Undergrad / Grad
Duration: 33:34
How to Adjust the Eyepiece and Camera
Nico Stuurman explains how to adjust the eyepiece and camera so when the microscope is switched between the two, the camera is immediately in focus. (Talk recorded in April 2012)
Audience:
- Researcher
Duration: 5:07
Light Sources for Microscopy
Nico Stuurman describes different light sources for microscopy and shows a few examples of light sources that are currently used in microscopes. (Talk recorded in April 2012)
Audience:
- Researcher
Duration: 7:19
Measuring a Point Spread Function
Nico Stuurman demonstrates the technique for measuring a point spread function by imaging a bead. (Talk recorded in April 2012)
Audience:
- Researcher
Duration: 15:16
