Lectures
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Introduction to Fluorescence Microscopy
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Fluorescent Probes: Organic Dyes and Quantum Dots
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Fluorescent Proteins and the Story Behind GFP
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Fluorescent Protein Indicators
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Optical Sectioning and Confocal Microscopy
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Two-Photon Microscopy
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Light Sheet Sectioning
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Overview and Stimulated Emission Depletion (STED) Microscopy
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Total Internal Reflection Fluorescence (TIRF) Microscopy
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Super-Resolution: Localization Microscopy
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Super-Resolution: Structured Illumination Microscopy (SIM)
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Measuring Dynamics: Photobleaching and Photoactivation
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Measuring Dynamics: Fluorescent Speckle Microscopy
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Förster Resonance Energy Transfer (FRET) Microscopy
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Fluorescence Lifetime Imaging Microscopy
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Summary: Designing a Fluorescence Microscopy Experiment
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Dual-View Inverted Selective Plane Illumination (diSPIM)